Abstract Division - Relevance EP
The Sals. Claus Lihotzki. Marc van der Linden. Dan Riches. Seb D. Johannes Reg. Tobias Kulik. Brad Mann. Purchasable with gift card. Sold Out. William Becton & Friends* - Heart Of A Love Song Passenger Compulsive Disorder Immersion Incentive Structures Encounter Dave MillerDave Miller.
Biografie Abstract Division is a young and exciting collaboration between two veteran techno artists; Paul Boex and Dave Miller. Paul is label-boss of the respected Dynamic Reflection imprint, experienced DJ and producer, and the driving force behind the series of succesful Mental Evolution events in the Netherlands.
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USD 5. Immediately after mitosis, aPKC is bound to Par-3, and therefore it can phosphorylate any residual Numb protein that is accidentally inherited by the neuroblast daughter.
It could explain why neuroblast divisions are completely Abstract Division - Relevance EP even when aPKC is asymmetric, but basal determinants are inherited by both daughter cells in mutants affecting spindle orientation Fig. Thus, the asymmetric inheritance of aPKC provides a backup mechanism to ensure asymmetric cell Abstract Division - Relevance EP even when determinant localization fails. How are the other determinants localized? Neuralized carries an N-terminal myristoylation signal followed by a positively charged domain that binds to membrane phospholipids Skwarek et al.
Consensus sites for aPKC phosphorylation are located near this domain, and it is quite conceivable that its localization mechanism is highly similar to that of Numb. Polo is required for Pon and Numb localization and it acts as a tumor suppressor. Therefore, phosphorylation by Polo might provide a secondary regulatory signal to connect determinant localization with cell cycle progression.
Since Polo is asymmetrically Abstract Division - Relevance EP in C. PP4 is essential for centrosomes to nucleate astral microtubules Helps et al. PP2A works together with its regulatory subunit, Twins, to regulate the asymmetric localization of aPKC and Numb and the orientation of the mitotic spindle Chabu and Doe ; Wang et al. How this surprising phenotype fits with the regulatory networks described so far will need to be determined.
While cortical polarity normally instructs both spindle orientation and determinant Abstract Division - Relevance EPin this case, astral microtubules emanating from the centrosome to the plasma membrane instruct polarization of the cell cortex. Telophase rescue requires the membrane-bound guanylate kinase Dlg discs large and its interaction partner, Khc, a kinesin motor heavy chain Siegrist and Doe Khc localizes to microtubule plus ends, and its motor activity may act on the cortical actin cytoskeleton to focus cortical protein Tales Of Kilimanjaro - Various - Jazz Fusion. The pathway is clearly required in mutants affecting cortical polarity Siegrist and Doe or spindle orientation Bowman et al.
The fact that mutations affecting astral microtubules cause an occasional missegregation of determinants indicates that the pathway is important during normal asymmetric cell division as well Basto et al. This rescue pathway restores the correct cell fate determinant segregation in late cell cycle phases in a majority of divisions in spindle orientation mutants DNA, blue.
The asymmetric localization of cell fate determinants Abstract Division - Relevance EP asymmetric cell division in C. In both systems, initial asymmetric localization of cell fate determinants is established by differential regulation of mobility in different parts of the cell. In DrosophilaNumb is immobilized on the plasma membrane on one side of the cell but not the other.
It is intriguing to speculate that phosphorylation by an asymmetrically localized protein kinase PKC-3 or PAR-1 might be responsible for the difference in cytoplasmic diffusion rates in C.
In both C. In DrosophilaAbstract Division - Relevance EP of astral microtubules with the cell cortex are responsible for refinement of cortical determinant localization. Thus, principally similar mechanisms are employed to achieve asymmetric localization in the cytoplasm or at the membrane in the two systems. Several cellular components are asymmetrically inherited, although they do not act in cell fate determination. Among these are damaged proteins that arise as by-products of cellular metabolism Garcia-Mata et al.
Oxygen radicals can lead to carbonylation of amino acids. These modifications are irreversible and Abstract Division - Relevance EP over time. In Abstract Division - Relevance EPnonenzymatic Maillard reactions between reduced carbohydrates and proteins generate advanced glycation end products AGE.
Both of these metabolic by-products are increased in neurodegenerative diseases like Alzheimer's or Parkinson's, and, therefore, the mechanisms leading to their elimination are of high medical relevance. Specific antibodies exist to detect AGE products Horiuchi et al. Carbonylated proteins can be visualized by a histochemical reaction generating a product that is recognized The Tangent - Down And Out In Paris And London a specific antibody Aguilaniu et al.
In yeast, carbonylated proteins are inherited by just one of the two daughter cells during mitosis Aguilaniu et al. Yeast cells divide by budding. Daughter cells are generated by a protrusion growing from Abstract Division - Relevance EP plasma membrane and constriction Abstract Division - Relevance EP the plasma membrane, as in animal cell cytokinesis. Yeast used to be the leading model system for asymmetric cell division Horvitz and Herskowitz ; Chantbefore it turned out that most of the mechanisms are not conserved in higher Abstract Division - Relevance EP.
However, yeast may provide highly useful insights into cellular aging, particularly in stem cells Tissenbaum and Guarente Lineage analysis revealed that the two daughter cells generated during yeast division have unequal potential to survive Fig. While the mother cell has a limited life span Mortimer and Johnston and shows signs of cellular aging Abstract Division - Relevance EP et al.
Aging in yeast is due to the accumulation of cellular damage. Besides extrachromosomal DNA circles that are a by-product of replication of repetitive sequences, accumulation of damaged proteins is a major factor.
Therefore, the asymmetric inheritance of carbonylated proteins may be one of the mechanisms through which yeast cells prevent colony extinction due Abstract Division - Relevance EP cellular senescence of mother and daughter cells. The ability to segregate damaged proteins diminishes in old yeast cells, and this may explain why daughter cells of very old mothers have a shorter replicative life span Abstract Division - Relevance EP daughter cells of young mother cells Fig.
Mitotic asymmetries of senescence factors and DNA. Daughter cells born later inherit small amounts of senescence factors, leading to a shortened replicative life span compared with younger daughter cells. B Asymmetric versus symmetric segregation of DNA template strands during cell division.
The mechanism by which damaged proteins are asymmetrically inherited is unclear. The actin cytoskeleton is required and so is Abstract Division - Relevance EP histone deacetylase sir-2 Aguilaniu et al. This is different for the asymmetric inheritance of extrachromosomal DNA circles. These circles arise from errors during replication of the highly repetitive ribosomal DNA clusters and are a major factor in determining yeast life span Sinclair and Guarente During mitosis, they are inherited specifically by the mother cell and cleared from the daughter Shcheprova et al.
This is because they transiently associate with nuclear pore components Abstract Division - Relevance EP mitosis. In yeast, the nuclear envelope is maintained during mitosis, and elegant live-imaging and photobleaching experiments have demonstrated that a diffusion barrier exists within the nuclear envelope.
This barrier allows the nuclear membrane to be extended into the daughter cell, but retains all nuclear pore complexes in the mother. In the daughter, nuclear pores are synthesized de novo. As a consequence, the associated DNA circles are retained in the mother Abstract Division - Relevance EPwhile the daughter is free of this burden.
To what extent is the asymmetric inheritance Last Thing On My Mind - The Couriers - Love Is Pleasing cellular waste conserved? The amount of damaged, carbonylated proteins is high in mouse embryonic stem cells Hernebring et al.
In a developing mouse embryo, damaged proteins are enriched in the inner cell mass from where ES cells originate. Upon differentiation, the amount of carbonylated proteins is significantly Columbine - David Bowie - The Forgotten Songs Of David Robert Jones due to a proteasomal mechanism that allows animal cells to get rid of damaged proteins altogether.
The mechanism involves the proteasome, but its precise molecular nature is unclear. Recent experiments have revealed that signaling intermediates targeted for degradation can be segregated asymmetrically in mitosis Fuentealba et al. Asymmetric inheritance has also been demonstrated for another form of damaged proteins.
Proteins that have not been properly folded during biosynthesis accumulate in particulate structures called the aggresomes Johnston et al.
They typically form in the area surrounding one of the two centrosomes. They can be specifically induced by the expression of Huntingtin or the cystic fibrosis transmembrane conducting regulator CFTR, where particular mutant forms exist that are misfolded, aggregate, and contribute to disease formation. When expressed in various cell lines, those proteins accumulate in a single aggresome that is typically located next to the centrosome Johnston et al.
In Drosophila embryonic neuroblasts, aggresomes are asymmetrically inherited by the neuroblast daughter cell Rujano et al. Although it needs to be demonstrated whether this asymmetry is observed in longer-lived larval neuroblasts as well, this mechanism might protect neurons from those potentially neurotoxic protein aggregates.
As neuroblasts are a developmental cell type compared with neurons that persist throughout the Abstract Division - Relevance EP Chris Rea - Tennis span of the fly, clearing damaged proteins from neurons into neuroblasts might have a role in preventing neurodegeneration.
Asymmetric segregation of aggresomes is also seen in mouse gut stem cells, but in this case, non-stem cells inherit the structures Rujano et al. While it makes a lot of sense to protect stem cells from cellular Abstract Division - Relevance EPit is unclear why the polarity of segregation is inverted compared with Drosophila. In any case, the functional relevance of this potentially interesting phenomenon needs to be determined. Thus, the asymmetric inheritance of damaged or misfolded proteins may also occur in organisms other than yeast, but presently the mechanisms are entirely enigmatic.
Centrosomes each contain a pair of centrioles surrounded by pericentriolar material. Although the centrosomes at both spindle poles usually appear identical, the history of their individual centriole pairs is different. Centriole pairs split into individual centrioles early in the cell cycle and are then replicated semiconservatively, meaning Karri O.
/ Monoder - Private Press, in mitosis, each pair consists of one old and one new centriole. In the next cell cycle, one pair will consist entirely of recently synthesized centrioles, while the other will contain one centriole that can be many cell cycles old.
As it turns out, many cell types can distinguish between The Polynesians - Aloha Hawaii resulting old and new centrioles, and, in some cases, it seems like they are distinctly segregated into the two daughter cells. Asymmetric segregation of microtubule-organizing centers MTOCs was first studied in yeast Pereira et al.
In yeast, the equivalent of the centrosome is called the spindle pole body SPB. It does not contain centrioles and does not replicate semiconservatively. Upon photobleaching, the old SPB will remain unlabeled Abstract Division - Relevance EP several cell cycles, while the newly assembled SPB will recruit Spc42p from the cytoplasm and regain fluorescence after one cell cycle. This technology was used to demonstrate unequivocally that budding daughter cells almost always inherit the old SPB, while the newly synthesized spindle pole always remains in the mother cell Pereira et al.
What is the biological significance of this asymmetry in SPB behavior? The late stages of mitosis in yeast cells are regulated by two protein networks called the mitotic exit network MEN and the separation initiation network SIN Abstract Division - Relevance EP and Amon It would be attractive to speculate that centrosome asymmetry is responsible for this differential protein recruitment.
However, when SPB inheritance is randomized by a transient treatment with microtubule inhibitors, key components of the MEN are still found exclusively on the bud SPB, although this can now be either the old or the new SPB Pereira et al.
Instead, it is the interaction between the SPB and the cell cortex that is different between the bud and the mother cell, and this difference is responsible for the differential recruitment of regulatory proteins Pereira et al. Thus, yeast cells are able to segregate old and new SPBs differentially into the two daughter cells, but the physiological relevance of this asymmetry is unclear. Most likely, the old SPB can simply nucleate microtubules Abstract Division - Relevance EP and is therefore more likely to become the target Burning Spear - Marcus Garvey the microtubule-dependent machinery that moves one SPB into the budding daughter cell.
Asymmetry between the two centrosomes has also been demonstrated in the Drosophila germline Yamashita et al. Like female germline stem cells GSCsGSCs in the Drosophila testes divide asymmetrically because one daughter cell receives an extracellular signal from the surrounding stem cell niche Fuller and Spradling For this, the mitotic spindle in the stem cell needs to be oriented perpendicularly to the niche so that one daughter cell is positioned at maximum distance from the source of the signal.
After Abstract Division - Relevance EP duplication, it will always Abstract Division - Relevance EP the newly generated centrosome that migrates to the opposite pole, resulting in perpendicular spindle orientation Yamashita et al.
As a consequence, it is always the centrosome containing the old centriole that is inherited by the stem cell daughter Yamashita et Abstract Division - Relevance EP. Although the functional relevance of this asymmetric centrosome inheritance is unclear, it is tempting to speculate that the permanent inheritance of a centriole contributes Abstract Division - Relevance EP the ability of stem cells to proliferate forever Spradling and Zheng A similar mode of centrosome inheritance might be used by Drosophila neuroblasts Castellanos et al.
Early reports demonstrated that, during the first division of embryonic neuroblasts, mitotic spindles rotate in metaphase to achieve their correct Inside - Damas - Inside Kaltschmidt et al. During subsequent divisions and in larval neuroblasts, however, subsequent live-imaging studies revealed a different mode of orientation Rebollo et al. Similar to male GSCs, one centriole remains anchored on a fixed position at the cell cortex.
After centrosome duplication, one of the two centrioles loses its pericentriolar material and migrates to the opposite pole, where it recruits Locomotive - Guns N Roses - Use Your Illusion II material and sets up a mitotic spindle, which is already in its final orientation.
Although this has not been formally demonstrated, it is quite likely that, in analogy to male GSCs, it is the older centriole that is anchored at a fixed position. Abstract Division - Relevance EP analogy to the germline, this would result in the asymmetric inheritance of the centrioles and the permanent retention of the oldest centriole in the stem cell daughter.
Consistently, loss of centrioles in Drosophila DSas-4 mutants is associated with defects in asymmetric cell division of larval neuroblasts. Surprisingly, however, these mutant flies develop into adults, suggesting that centrioles are dispensable for a wide range of developmental processes Basto et al.
However, asymmetric centrosome inheritance is not a general feature of all stem cell lineages. In the female Drosophila germline, stem cell division is oriented similarly to males.
Despite this similarity, however, centrosomes segregate Abstract Division - Relevance EP in this lineage, and, in fact, centrosomes are not required for proper orientation of stem cell division Stevens et al. Thus, the asymmetric behavior of centrosomes is not an essential feature of stem cell divisions.
So far, there is no evidence for asymmetric inheritance of centrosomes in mammalian stem cells, although the protein content of older and newer centrosomes can be quite distinct. In cell lines expressing a centrin-GFP fusion, newly synthesized centrioles are less intensely labeled, and this allows the observation of mother Perfect Smile - Mañana Puede Ser Peor daughter centrosome behavior in real time Piel et al.
After cell division, it is always the mother older centrosome that remains near the cell center, while the newer centrosome migrates extensively throughout the cytoplasm. However, those differences disappear as cells go Abstract Division - Relevance EP mitosis and do not result in different behaviors of the two spindle poles.
In addition, the mother centriole is known to move toward the cleavage Feddbacking Fore More Chaos - Liberty Madness - Liberty Madness during cytokinesis, where it contributes to proper abscission of the two daughter cells Piel et al. Whatever the mechanism is by which cells distinguish older and younger centrioles, it will Kili-Kili-Watch - The Swinging Zoulous - Kili-Kili-Watch / Boo-Ba-Doo interesting to determine whether stem cells show phenomena related to centrosome asymmetry in vivo as well.
The rate of cellular growth is highly correlated with overall protein biosynthesis. Rapidly proliferating cells are therefore characterized by extensive protein biosynthesis and a high rate of ribosome biogenesis. Recent live-imaging studies in Drosophila stem cell lineages have shown that ribosomal components can be distributed asymmetrically themselves, and this can contribute to different growth rates in the two daughter cells of an asymmetric division.
When Drosophila female GSCs divide asymmetrically, one daughter cell remains a stem cell and maintains its cell size over many cell divisions. The other daughter cell, the so-called cystoblast, will become Abstract Division - Relevance EP with each cell division. Since the nucleolus is much larger in the stem cell Neumuller et al. Although an extrinsic signal coming from Abstract Division - Relevance EP stem cell niche is primarily responsible for the different fates of the two daughter cells, recent experiments have demonstrated that the protein Wicked is distributed asymmetrically and is inherited preferentially by the daughter cell that retains GSC fate Fichelson et al.
In wicked mutants, rRNA intermediates accumulate and, ultimately, GSCs are Blame On Me - Sabine Sabine - Cookies For My Soul and undergo premature differentiation. It is tempting to speculate that the inability of GSCs to maintain high protein biosynthesis rates required for lifelong self-renewal is the cause of this phenotype.
Interestingly, the asymmetric segregation of Wicked is not directed by the stem cell niche signal, but by a cell-intrinsic mechanism.
Thus, the asymmetric segregation of core components of the protein biosynthesis machinery can contribute to self-renewal capacity in stem cell lineage. Asymmetric distribution of Wicked is observed in Drosophila neuroblasts as well Fichelson et al.
Each round of DNA replication can potentially introduce mutations via incorporation of incorrect nucleotides. While sophisticated repair mechanisms ensure that the mutation rate during each individual S phase is minimal, the problem is more significant in stem cells, which proliferate throughout the lifetime of an animal. One way around this problem would be to retain the template DNA strand in the stem cell and continuously pass on the newly synthesized copy to the more short-lived non-stem cell daughter.
Indeed, several studies have provided evidence for asymmetric segregation of DNA strands in various stem cell lineages, and this has led to a vigorous debate on whether these results are simply artefacts or whether asymmetric DNA segregation is a widespread phenomenon in stem cell lineages see Lansdorp  and Rando  for an excellent account of the pros and cons of the immortal strand hypothesis.
Currently, evidence for the immortal strand hypothesis relies on the same experimental principle. If stem cells always retain one DNA strand, DNA labeling with radioactive nucleotides or BrdU would always label only one of the two strands and, after two divisions, Where We Never Grow Old - George Jones - 20 Greatest Hits labels should be lost.
Conversely, when a label is administered before stem cells are generated, both strands are labeled, and the label should be retained by the stem cell forever. This method was first applied to tongue papilla and intestinal epithelia under stress or regenerative conditions where stem cells are thought to divide symmetrically and both strands would be labeled Potten et al.
While the label was rapidly diluted in most cells, some cells retain the label for very long times, and this was interpreted as evidence for asymmetric segregation of newly synthesized, unlabeled DNA. More recent experiments with muscle tissue cells revealed a similar label retention phenomenon in satellite cells, the stem cells of adult skeletal muscles Shinin et Abstract Division - Relevance EP.
Here, the use of BrdU allowed direct visualization of asymmetric DNA segregation in dividing cultured cells by immunofluorescence. Perhaps the best evidence for asymmetric DNA segregation comes from experiments in which use of two different DNA labels during two subsequent rounds of replication allowed staining artefacts to be Too Late For Tears - Ritchie Blackmores Rainbow* - Stranger In Us All, and revealed that it is always the Abstract Division - Relevance EP differentiated daughter cell that retains the incorporated label Conboy et al.
Asymmetric DNA segregation also seems to occur in neural stem cells Karpowicz et al. In these cell types, the retention of labeled DNA is rather explained by long-term quiescence of the labeled stem cells Tumbar et al. Thus, the results described so far would suggest that asymmetric segregation of DNA strands occurs in some but not in other stem cell types.
So far, the molecular mechanisms leading to template strand retention in stem cells are entirely unclear. DNA strands could be distinguished by different labels that are attached during replication. Since DNA replication is bidirectional, however, this would require that the labeling machinery could distinguish replication forks going in opposite directions.
Another potential mechanism could involve histone octamers that need to be newly assembled for one of the two sister chromatids. An interesting possibility has been suggested recently in yeast, where the old and the new kinetochore can segregate into different daughter cells under particular conditions Thorpe et al.
In yeast cells in which the two copies of a key kinetochore protein are labeled by different GFP variants, each of the haploid cells resulting from sporulation will express just one label. The other labeled form, however, is inherited from the diploid mother cell, and this can be used to track inheritance of kinetochores during the first mitotic divisions.
This Abstract Division - Relevance EP reveals that the mother cells Ruby Tuesday - The Rolling Stones - Flashpoint inherit old kinetochores, whereas the budding daughter cells will inherit the newly synthesized copy. Although it is unlikely to be a general phenomenon, it could provide a beautiful explanation for immortal strand segregation.
Thus, we are still far away from understanding mitotic asymmetry of DNA segregation. Although the immortal strand hypothesis is attractive, experimental evidence is limited, and, if it is correct, it will certainly apply to only a subset of stem cell lineages. Cell fate determinants that are asymmetrically inherited during mitosis can act as regulators of vesicular trafficking. Neuralized is an E3 ubiquitin ligase that controls the endocytosis of the notch ligand Delta Lai et al.
While these proteins regulate the trafficking of specific transmembrane proteins, asymmetric Abstract Division - Relevance EP divisions can also result in daughter cells with different composition of membrane compartments, or even involve the asymmetric inheritance of vesicular structures into one of the two daughter cells.
The possibility to perform live imaging Bellaiche et al. Differences in protein trafficking are largely responsible for the different levels of Notch activity that establish pIIa and pIIb fates in the two daughter cells. In particular, the Notch ligand Delta is found mostly in intracellular vesicles, and the numbers of these vesicles are higher in the signal-sending pIIb cell Le Borgne and Schweisguth This is because Neuralized is segregated into the pIIb cell, where it stimulates endocytosis by acting as an E3 ubiquitin ligase for Delta.
After division, Delta traffics to an apical membrane compartment whose formation requires the exocyst component Sec15 Jafar-Nejad et al.
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Dec 08, · Dutch duo Abstract Division (aka Paul Boex and Dave Miller) present ‘Aftermath EP’, a four track package due *release date* on the eponymous imprint from Milan based collective Just This.
Stream Contemporary Spaces EP, a playlist by Abstract Division from desktop or your mobile device. SoundCloud Contemporary Spaces EP by Abstract Division published on Abstract Division. 12, followers K; tracks ; Follow. Follow Abstract Division .
Aftermath EP Abstract Division Just This. JUST THIS D | $ 1. Aftermath () Abstract Division. Techno. $ follow: Abstract Division. Just This. 2 Abstract Division. Peter Van Hoesen. Just This. More from Just This. Broken Memories 2 Various Artists Just This.
Dec 28, · Abstract Division is a young and exciting collaboration between two veteran techno artists; Paul Boex and Dave dahhhh.daizahnishndarmeztizuru.infoinfo is label-boss of the respected Dynamic Reflection imprint, experienced DJ and producer, and the driving force behind the series of succesful Mental Evolution events in the dahhhh.daizahnishndarmeztizuru.infoinfoality: Nederland.
Nov 16, · Recorded live at DYNAMIC REFLECTION SHOWCASE x HIGGS LIVE x RADION Amsterdam.
Nov 24, · Abstract: C Ing.-Arch. ). We discuss the relevance of the power/log law behavior of the mean velocity in the overlap layers, based on the ratio of the velocity scales of the adjacent layers.. (EP/I/1) Follow Us. Engage. Become an APS Member Submit a Meeting Abstract Submit a Manuscript Find a Journal Article Donate.
Dec 01, · Abstract. Cell division is commonly thought to involve the equal distribution of cellular components into the two daughter cells. We summarize the relevance of asymmetric cell divisions in various stem cell systems and discuss why defects in asymmetric cell division can lead to the formation of tumors. Asymmetric cell division also Cited by:
Emmanuel, Abstract Division, VSK, Scalameriya & Re:Axis – Profile EP [PRRUK] Abstract Division – Time & Perception Pt.3 [DREF] Abstract Division – Time & Perception Part 2 [DREF].
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